In Vitro Generation of Pancreatic Pseudo-islets Using Free-standing Mesh Patterned Cellular Hydrogel
نویسندگان
چکیده
This paper reports a simple and direct method to fabricate mesoscopic hydrogel constructs for size controlled pseudo-islets with MIN6 cells, a pancreatic β-cell insulinoma, via lamination of free-standing mesh patterned cellular hydrogel. The MIN6-laden hydrogels with three different micro-patterns were simply fabricated, free-standing cultured, and functionally analyzed to examine the effect of the microscale hydrogel patterns on pancreatic cellular growth. The presented system can be applied to an in vitro model for investigating cell and tissue morphogenesis in three dimensions (3D) and biofabrication of tissue constructs with microscale control of 3D cellular geometry, which could have great potential for the basic platform of functional pancreatic cellular assay.
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